Optical Biology Core

Overview of Services

OBC has multiple instruments applicable to biological applications including a Zeiss LSM 780 with 2 photon and incubation, LSM 900 with Airyscan 2, Zeiss Z1 Mesoscale lightsheet, Elyra 7 with Lattice SIM and SMLM, LSM 700, and a Leica SP8 confocal microscope, as well as additional facilities to support Multi-Dimensional Microscopy (MDM). Multiple workstations including Imaris 3/4D Image Visualization and Analysis Software, Imaris Stitcher, Arivis, ZEN Blue for Airyscan processing and ZEN Black for SIM processing, and Huygens Deconvolution are available for use in the OBC. 

The NLOM Lab at the Beckman Laser Institute and Medical Clinic at UC Irvine develops and uses modern biophotonics technologies such as nonlinear optical microscopy for basic research, pre-clinical and clinical applications. One of our major goals related to clinical applications is to use this technology as a non-invasive imaging tool to visualize and understand the microscopic and molecular processes that underlie skin biology. We collaborate closely with the Dermatology Department at UCI to overcome the challenging task related to the clinical translation of multiphoton microscopy. Our Lab is an imaging shared resource for the Chao Family Comprehensive Cancer Center and the Skin Biology Research Center at UCI. Shared instrumentation: Leica SP8 Falcon + coherent anti-Stokes Raman Scattering (CARS)

This is a commercial imaging platform customized to feature the following modalities: confocal fluorescence and two-photon excited fluorescence (TPEF) microscopy, second harmonic generation (SHG), CARS and fluorescence lifetime microscopy. 

It is very important to the continued funding of the Chao Family Comprehensive Cancer Center (CFCCC) that the productive collaboration of CFCCC-supported Shared Resources is acknowledged in publications and presentations, and that resulting publications are processed by the National Institutes of Health Manuscript Submission (NIHMS) to obtain a PubMed Central reference number (PMCID), per NIH Public Access Policy.

 

For all publications using any of the resources from the OBC, be sure to include the following statement in the acknowledgments:

This study was made possible in part through access to the Optical Biology Core Facility of the Developmental Biology Center, a shared resource supported by the Cancer Center Support Grant (CA-62203) and Center for Complex Biological Systems Support Grant (GM-076516) at the University of California, Irvine.”--

Please visit the CFCCC website to learn more about Acknowledging the Cancer Center Support Grant (CCSG) and Shared Resources.

 

_________________________________________________________________________________________

The 20th Annual Advanced Imaging Methods Workshop held in person this year at UC Berkeley

January 24 – 26, 2023

Register Here

The 20th Annual Advanced Imaging Methods Workshop is a 3-day event focused on new and emerging optical microscopy techniques and their applications with a special focus on time-resolved techniques hosted by the CRL Molecular Imaging Center at UC Berkeley.

This workshop brings together internationally-renowned researchers in a broad variety of fields including biology, chemistry, physics, engineering, probe design, cellular biology, neuroscience, optics, metabolism and more. Past speakers have included six Nobel Laureates: Roger Tsien, WE Moerner, Stephen Hell, Eric Betzig, Steven Chu, and Carolyn Bertozzi.

In addition to invited talks from leaders in the field, AIM2023 will include real lab demonstrations on campus, sponsor demonstrations, software tutorials, tours of UC Berkeley state-of-the-art imaging facilities, and a special reception at the outdoor lounge (weather permitting). This year will also bring back the popular poster session with prizes for student presenters. AIM includes gourmet lunch on-site each day, coffee breaks, and plenty of opportunities for interactions among participants.

_____________________________________________________________________________________________

Direct Link to the UCI Flow Cytometry Core HERE

Leadership

Dr. Adeela Syed|Project Scientist and Manager of Optical Biology Core 

Dr. Rahul Warrior|Associate Professor and Director of Optical Biology Core

Dr. Mihaela Balu|Associate Researcher of NLOM

 

SUPPORT FOR CONFOCAL USERS

Adeela Syed PhD, OBC Manager

Hours: ~9:30am to 4:00pm and by appt.

You can contact Adeela via email (adeelas@uci.edu), or phone (949-824-3856)

 

SUPPORT FOR NONLINEAR OPTICAL MICROSCOPY LAB 

Mihaela Balu, PhD, Associate Researcher

Hours: ~9:30am to 4:00pm and by appt.

You can contact Mihaela via email (mbalu@uci.edu), or phone (949-824-8797)

 

Links and Resources

  1. Optical Biological Core Facilty
  2. Nonlinear Optical Microscopy Lab

Contacts

Name Role Phone Email Location
Dr. Adeela Syed
Project Scientist and Manager of Optical Biology Core
 
949-824-3856
 
adeelas@uci.edu
 
4443 McGaugh Hall
 

Available Equipment and Resources

OBC Microscopes (5)
OBC Workstations (3)